Cloning and sequence characterization of a partial Piper colubrinum phytoene desaturase (PcPDS) gene homologue for virus-induced gene silencing studies
In the present study, we report the partial cloning and characterization of Phytoene desaturase (PDS) gene from the plant Piper colubrinum, a distant relative of Piper nigrum (Black pepper). P. colubrinum is the only species reported to be totally immune to Phytophthora capsici infection. PDS gene is widely used as a visual reporter gene for functional genomics studies in various plant species. We have amplified a 1 kb PDS fragment from P. colubrinum cDNA using degenerate primers and cloned it into pCR®8/GW/TOPO® vector. Sequencing and further phylogenetic analysis confirmed the sequence as Phytoene desaturase. The PDS gene fragment was re-amplified using gene specific primers and sequence was confirmed for its further use in virus-induced gene silencing studies.
Volume & Issue : Vol 1 No 1 (2015)
Page No.: 113-117
Published on: 2015-01-31
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This work is licensed under a Creative Commons Attribution 4.0 International License.
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